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Comparison of mRNA expression levels of TLR4, RANK, <t>RANKL,</t> <t>and</t> <t>OPG</t> in lung tissues of mice in each group. The mRNA levels were detected with real‐time quantitative PCR. (A) Level of RANKL mRNA. (B) Level of OPG mRNA. (C) Level of RANK mRNA. (D) Level of TLR4 mRNA. *** p < 0.001, **** p < 0.0001.
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Comparison of mRNA expression levels of TLR4, RANK, RANKL, and OPG in lung tissues of mice in each group. The mRNA levels were detected with real‐time quantitative PCR. (A) Level of RANKL mRNA. (B) Level of OPG mRNA. (C) Level of RANK mRNA. (D) Level of TLR4 mRNA. *** p < 0.001, **** p < 0.0001.

Journal: Immunity, Inflammation and Disease

Article Title: RANKL Attenuates Sepsis‐Associated Acute Lung Injury Through the OPG/RANKL/RANK/TLR4 Pathway

doi: 10.1002/iid3.70356

Figure Lengend Snippet: Comparison of mRNA expression levels of TLR4, RANK, RANKL, and OPG in lung tissues of mice in each group. The mRNA levels were detected with real‐time quantitative PCR. (A) Level of RANKL mRNA. (B) Level of OPG mRNA. (C) Level of RANK mRNA. (D) Level of TLR4 mRNA. *** p < 0.001, **** p < 0.0001.

Article Snippet: After that, the pre‐diluted primary antibodies against OPG (cat# DF6824; Affinity Bioscience), RANKL (cat# 23408‐1‐AP; Proteintech Group Inc.), RANK (cat# ab222215; Abcam), TLR4 (cat# 19811‐1‐AP; Proteintech Group Inc.), and β‐actin (cat# TDY051; Tiandeyue Biotechnology Co. Ltd) were added and probed overnight at 4°C.

Techniques: Comparison, Expressing, Real-time Polymerase Chain Reaction

Comparison of protein expression levels of TLR4, RANK, RANKL, and OPG in lung tissues of mice in each group. The protein levels were detected with Western blot analysis. (A) Representative Western blot results. (B) The relative protein level of RANKL. (C) The relative protein level of OPG. (D) The relative protein level of RANK. (E) The relative protein level of TLR4. * p < 0.05, **** p < 0.0001.

Journal: Immunity, Inflammation and Disease

Article Title: RANKL Attenuates Sepsis‐Associated Acute Lung Injury Through the OPG/RANKL/RANK/TLR4 Pathway

doi: 10.1002/iid3.70356

Figure Lengend Snippet: Comparison of protein expression levels of TLR4, RANK, RANKL, and OPG in lung tissues of mice in each group. The protein levels were detected with Western blot analysis. (A) Representative Western blot results. (B) The relative protein level of RANKL. (C) The relative protein level of OPG. (D) The relative protein level of RANK. (E) The relative protein level of TLR4. * p < 0.05, **** p < 0.0001.

Article Snippet: After that, the pre‐diluted primary antibodies against OPG (cat# DF6824; Affinity Bioscience), RANKL (cat# 23408‐1‐AP; Proteintech Group Inc.), RANK (cat# ab222215; Abcam), TLR4 (cat# 19811‐1‐AP; Proteintech Group Inc.), and β‐actin (cat# TDY051; Tiandeyue Biotechnology Co. Ltd) were added and probed overnight at 4°C.

Techniques: Comparison, Expressing, Western Blot